Remote Sensing (RS) and Geographical Information System (GIS) were applied and map was generated. 56 unbaited biconical traps were deployed in various ecosystems assumed to habituate tsetse fliesand coordinates of the sites where traps were deployed were recordedusing Global Positioning System (GPS). A 2-year- study was carried out to spot spatial distribution of G.f. Studies on the spatial distribution of tsetse flies are imperative for the control of trypanosomiasis in the endemic foci. Glossina fuscipes fuscipes act as tsetse vectors of HAT in Kajo-keji County (KKC) South Sudan. Tsetse flies are the sole biological vectors of Human African Trypanosomiasis (HAT) in sub-Saharan Africa.
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